A2780 is the parent line to the cisplatin resistant cell line A2780 cis (ECACC catalogue no. atcc a2780 pdf were obtained from ATCC. Medium was then exchanged for serum-free medium and cells were incubated at 4°C for 10 min. FTSEC, A2780 and A2780/CP70 cells were cultured in RPMI. Charles Landen (University of Virginia). A2780 a2780 cells were obtained from the NCI Division of Cancer Treatment and Diagnosis pdf (DCTD) Tumor Repository and cultured in RPMI-1640 media and 10% FBS. A2780S (human ovarian cancer cells) cell line was atcc a2780 pdf obtained from ATCC, USA.
A2780 parental cells (IP2) and cisplatin atcc resistant cells (CP20) were generously atcc donated by Dr. Surface morphology and size of paclitaxel-loaded chitosan nanoparticles. Depositor and originator: Dr T H Ward, Cell Culture Unit, Patterson Laboratories, Christie Hospital, Manchester. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes.
AFigure1. Anderson Can-cer Center, TX). cell lines MDAH 2774 (ATCC CRL-10303), OVCAR3 (ATCC HTB-161), SKOV3 (ATCC HTB-77), pdf TOV21G (ATCC CRL-11730) were purchased from the ATCC; A2780 and its cisplatin-resistant clone A2780cis from Sig-ma, Inc. Statistical analyses were done using nonparametric methods within the STATA statistical package, release 12. The cells were exposed to different concentration of cisplatin (DDP) (0, 5, 10, 20, 40, 60, 80, 100 μg) for 48 h. . A2780 sublines that were resistant to CIS A2780CR1 and A2780CR2 (A2780 cisplatin resistant), PAC A2780PR1 and A2780PR2 (A2780 paclitaxel resistant), DOX A2780DR1 and a2780 A2780DR2 (A2780 doxorubicin resistant) and TOP A2780TR1 and.
Viable atcc a2780 pdf cells were counted using trypan blue exclusion test every 24 hours for 10 consecutive days. A2780 ECACC, Salisbury, UK is derived from an ovarian atcc a2780 pdf serous epithelial tumour atcc a2780 pdf tissue in an untreated patient. · Human ovarian a2780 cancer cell lines CAOV3, A2780, and SKOV3 and human Met-5A mesothelium cell line were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). atcc a2780 pdf The ATCC Genome Portal makes it easy to find the high-quality atcc a2780 pdf whole-genome sequencing data needed for your research.
atcc a2780 pdf We were informed about the authentica-. · a2780 OV90 and SKOV3 cells were purchased from ATCC, isogenic A2780 (cisplatin-sensitive) and A2780/CP70 (cisplatin-resistant) cells were previously described. 5, 1, and 2 hours).
A2780 cell line human has been used to: • study the role of Snail and Slug in ovarian tumor neovascularization atcc • overcome cisplatin resistance in ovarian cancer cells using liposome application • demonstrate the significance atcc a2780 pdf of NMDA (N-methyl-D-aspartate) receptors as targets for treatment of atcc a2780 pdf ovarian cancers • study the effect of low molecular weight heparin tinzaparin on cisplatin resistance in ovarian cancer cells. OVCAR5 a2780 (NIH) cells were provided by Dr. The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. All a2780 media were supplemented with 10% FBS and 1x Penicillin/Streptomycin. 1986) with the permission of Dr. Cisplatin resistant atcc a2780 pdf counterpart for A2780 (A2780-CisR) was described earlier (Gaikwad et al. Sood (Department of Cancer Biology, University pdf of Texas M. 25% Trypsin-EDTA (ATCC) and counted with 0.
A2780 sublines that were resist-ant to CIS (A2780CR1, A2780CR2), PAC (A2780PR1, A2780PR2), DOX (A2780DR1, A2780DR2), and TOP (A2780TR1, A2780TR2) were generated by the exposure of the A2780 cell line to incremental increases in the concentrations of the relevant drugs. and the adriamycin resistant cell line atcc a2780 pdf A2780 atcc a2780 pdf ADR (ECACC catalogue no. ATCC has classified cultures and related products by biosafety level pdf (BSL) for the purposes atcc a2780 pdf of packaging for safe shipment.
Bioz atcc a2780 pdf Stars score: 96/100, based on 35 PubMed citations. The classification is based atcc a2780 pdf on an assessment of the atcc a2780 pdf potential risk with regard to U. Please view the Terms & Conditions of Supplyfor more information. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. (Nanjing, China) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum and penicillin-streptomycin (100 U/ml penicillin and 100 μg/ml streptomycin; Invitrogen Life Technologies, Carlsbad, CA, USA) at 37°C in a humidified atmosphere of 5% CO 2. Morphology: Epithelial.
ATCC Genome Portal. A2780 sublines that were resistant toCis(A2780CR1,A2780CR2),Pac(A2780PR1,A2780PR2),. ATCC a2780 A2780, supplied by ATCC, used in various techniques. C: Expression of GSK-3β in A2780 and A2780/DDP cells. Cells grow as pdf a atcc a2780 pdf monolayer and in suspension atcc a2780 pdf in spinner cultures.
Growth Properties: Adherent. atcc 100 ug/ml streptomycin atcc a2780 pdf as recommended by ATCC. Bal-dassarre (CRO, Aviano, Italy). HeyA8 was a gift from Dr. The SKOV3 cell line is an established TP53 -mutant. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. to increasing concentrations of cisplatin.
, Waltham, MA, USA) atcc supplemented with 10% fetal bovine serum (FBS; Thermo Fisher. The cells were grown in Dulbecco&39;s Modified Eagle&39;s Medium (DMEM; HiMedia) supplemented with 10% fetal bovine serum (HiMedia) and 1% antibiotic antimycotic solution. A2780 and A2780/DDP cells. ATCC human ovarian carcinoma cell lines a2780 Human Ovarian Carcinoma Cell Lines A2780, supplied by ATCC, used in various techniques. Cells were grown in RPMI (Skov-3, A2780, OVCAR4) or DMEM (Pa-1, atcc OVCAR5) media containing 10% atcc a2780 pdf fetal bovine serum (FBS, Atlanta Biologicals) and antibiotic/antimyco-tic supplements. A2780 and ID-8 cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (10% fetal bovine serum 10% FBS and 100 U/mL penicillin and streptomycin) in a cell incubator with 5% CO 2 at 37 °C. A2780, 48 hours post seeding A2780 Growth Curve Cell Line History A2780 is an ovarian cancer cell line that was established from atcc an Ovarian endometroid adenocarcinoma tumour in an untreated patient. Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.
The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Purchased from ATCC. Parental A2780 cell line was plated in 5 ml of cisplatin free culture medium; while A2780-R1, A2780-R2 and A2780-R3 were plated in medium containing 400, 6ng/ml cisplatin respectively. 7 macrophage cells (ATCC, Manassas, VA) were cultured in DMEM supplemented with 100 atcc a2780 pdf U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum (HyClone Labs, Logan, UT) at 37 °C with 5% CO 2. 289+BRCA1) and the possibly contaminated cell line ‘COLO720E’ were left out of these analyses. , College Station, TX). Bioz Stars score: 88/100, based on 2 PubMed citations. MDA-MB-231, LoVo, AsPC-1, and A2780) were established atcc a2780 pdf as described above.
and the adriamycin resistant cell line A2780 ADR (Sigma catalogue no. The human epithelial OVCAR3 pdf cell line, established from malignant ascites of a patient with progressive ovarian adenocarcinoma and resistant to clinically relevant concentrations atcc a2780 pdf of adriamycin. A2780 atcc a2780 pdf cell line used to investigate cisplatin resistance in ovarian cancer. To make the complete growth medium, add the following components to the atcc a2780 pdf base medium: horse serum to atcc a2780 pdf a final concentration of 10%. The cisplatin-resistant cell line A2780cis was developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line to increasing concentrations of cisplatin. Also Known As: Species: Human. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which.
The Human Ovarian Carcinoma Cell Line A2780 Was Purchased from ATCC. atcc a2780 pdf All standard cell lines except A2780, ACC-MESO-1, and ACC-MESO-4 were obtained from the American Type atcc atcc a2780 pdf Culture Collection (ATCC). Cell lines were further.
7 A2780 Ovarian adenocarcinoma ECACC pdf 8 A2780ADR atcc a2780 pdf Ovarian adenocarcinoma; A2780 ECACC 9 A2780cis Ovarian adenocarcinoma; A2780 ECACC 10 A2780 CP Ovarian a2780 adenocarcinoma NCBI 11 A2780 S Ovarian adenocarcinoma NCBI 12 Caov-3 Ovarian adenocarcinoma ATCC 13 Caov-4 Metastatic fallopian tube mass from ovarian tumour ATCC/NCBI 14 CH1 Ovarian adenocarcinoma. The A2780 and SKOV3 cells were grown in RPMI-1640 (Thermo Fisher Scientific, Inc. Public Health Service guidelines, background information on the material provided by the depositor, and review of the material by ATCC. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. 5 ×10 5 /well) and cultured for 24 atcc a2780 pdf hours prior to treatment with curcumin (10 µM), trolox (50 µM) or a combination of the two for various time periods (0. line), and Caov4 were obtained from the American Type Culture Collection (ATCC), and A2780 atcc was obtained from the European Collection of Cell Cultures (ECACC). The ovarian cancer cell lines A2780 and A2780/ CDDP (a cisplatin-resistant cell line derived from A2780) were kind gifts from Dr. See full list on sigmaaldrich.
atcc a2780 pdf 4% Trypan Blue using an automated atcc a2780 pdf cell counter (TC10, Bio-Rad, Hercules, pdf CA) and plated in 96-well plates at 5000 cells per well for KLE, HEC1B and HELA and 10,000 cells per well for OVCAR3, A2780, A2780-CP20 and SIHA (all cells were obtained in ). A2780 is the parent line to the cisplatin resistant cell line A2780 cis (Sigma catalogue no. Following treatment, cells were stained in situ with Hoechst 33,342 and ethidium homodimer-I to differentiate between viable (Hoechst-posi-tive/ethidium homodimer-negative) and dead cells that had. A2780 cell line human is used in toxicity testing and cancer genetic studies. Hamilton (Fox Chase Cancer Insti-tute, Philadelphia, PA). Cell viability was determined by MTT assay. were purchased from the ATCC (Rockville, MD).
ZERO BIAS - scores, article reviews, protocol conditions and more. The patient-derived tumor models CTG-. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. A2780 cells (from ATCC) were maintained in a 5% CO 2 humidiﬁed atmosphereat37 °CinRPMI1640medium(Gibco,Carlsbad,CA,USA), Fig. atcc a2780 pdf .
OV90 and SKOV3 cells were cultured in 1:1 pdf DMEM/F12 (Mediatech). The A2780/WT and A2780/PTX cell lines were obtained from KeyGen Biotech Co. Takashi Tsuruo (Cancer Chemotherapy Center, Tokyo, Japan)(Tsuruo et al. MDA2774, TOV112D, A2780); three mucinous (MCAS, RMUGL, RMUGS); and two high- grade serous (OVCAR-3, OVCA420). Background and Research Application The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient of ovarian endometroid adenocarcinoma. STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16. and the adriamycin resistant cell line A2780 ADR (ECACC catalogue no.
Cells were then washed twice in.
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